T4 DNA Ligase Thermo
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T4 DNA Ligase (5 U/µL) - Thermo Fisher ScientificThermo Scientific T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5'-phosphate and 3'-hydroxyl termini in duplex DNA or ... twT4 DNA Ligase (1 U/µL) - Thermo Fisher ScientificT4 DNA Ligase catalyzes the formation of phosphodiester bonds in the presence of ATP between double-stranded DNAs with 3 hydroxyl and 5 phosphate termini. twOPTIZYME™ T4 DNA Ligase, Fisher BioReagentsMilliporeSigma ™ Novagen ™ KOD Hot Start DNA. $308.00 / Each. Thermo Scientific™ T4 DNA Ligase, LC (1 U/µL). Catalog No. FEREL0016.Comparative analysis of the end-joining activity of several DNA ligases2017年12月28日 · This analysis revealed that T4 DNA ligase, the most common enzyme utilized for in vitro ligation, had its greatest activity on blunt- and ...T4 DNA ligase structure reveals a prototypical ATP-dependent ...2018年8月29日 · Bacteriophage T4 DNA ligase is the first ATP-dependent ligase ... the same buffer in a 2 kDa cutoff Slide-A-lyzer cassette (Thermo Fisher).DNA unwinding and inhibition of T4 DNA ligase by anthracyclines.The ability to alter DNA tertiary structure of ten anthracycline derivatives whose antitumor potency is known was studied by an assay that makes use of nicked ...圖片全部顯示Archaeal RNA ligase from thermoccocus kodakarensis for template ...Chlorella virus DNA ligase, T4 DNA ligase, and T4 RNA ligase 2 are all monomeric ... When a pre-treatment step with thermo-labile proteases is performed, ...single-molecule sequencing assay for the comprehensive profiling ...2018年5月8日 · This method has been used to profile the ligation of all three-base 5′-overhangs by T4 DNA ligase under typical ligation conditions in a single ...A high-efficiency method for site-directed mutagenesis of large ...2021年5月17日 · DNA ligation was performed in a 20 μL reaction mixture containing 5 μL phosphorylated samples and 5 U T4 DNA ligase (Thermo Scientific).
延伸文章資訊
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DNA損傷因素可以分為DNA內生性損傷與DNA外生性損傷,內生性損傷(如核苷酸鹼基 ... 單鏈斷裂重接, 一部分可由DNA連接酶(ligase)參與而完全修復;此酶在各類生物細胞中都 ...